Journal: Journal of Inflammation Research

Article Title: Association Between Ferritinophagy and M1 Macrophage Polarization During the Acute Phase of Childhood Allergic Asthma

doi: 10.2147/JIR.S569956

Figure Lengend Snippet: Analysis of Monocyte Subpopulations in PBMCs From Children With Allergic Asthma. ( A ) Schematic of the human sample workflow: Peripheral Blood Mononuclear Cells (PBMCs) were isolated from healthy controls (n = 15) and asthmatic children (n = 16) via centrifugation, followed by monocyte subpopulation analysis using flow cytometry. ( B ) Representative flow cytometry plots (gated on CD14/CD16) showing three monocyte subsets: C1 (classical monocytes, CD14++CD16-), C2 (intermediate monocytes, CD14++CD16+), and C3 (non-classical monocytes, CD14+CD16++). ( C ) Quantified proportions of each monocyte subpopulation in PBMCs from healthy controls and asthmatic children. ( D ) Serum IgE levels (measured by ELISA) in healthy controls and asthmatic children. *P<0.05, ***P<0.001, ns: not significant (P≥0.05).

Article Snippet: The concentration of total IgE in mouse serum was quantified using a commercial Mouse IgE ELISA Kit (Dakewe Biotech, China) according to the manufacturer’s instructions.

Techniques: Isolation, Centrifugation, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Journal of Inflammation Research

Article Title: Association Between Ferritinophagy and M1 Macrophage Polarization During the Acute Phase of Childhood Allergic Asthma

doi: 10.2147/JIR.S569956

Figure Lengend Snippet: Establishment and Validation of HDM-Induced Mouse Allergic Asthma Model. ( A ) Schematic of the HDM-induced allergic asthma model in mice. ( B ) Airway responsiveness (Rn) to increasing methacholine concentrations in PBS- and HDM-treated groups (n = 5 per group). ( C ) Serum IgE levels by ELISA in PBS or HDM sensitized and challenged mice (n=13 per group). ( D ) Representative H&E-stained lung tissue sections (scale bars: 200 μm and 50 μm, respectively). ( E ) Quantified inflammatory scores from H&E staining. ( F ) Representative PAS-stained lung tissues sections (scale bars: 200 μm and 50 μm, respectively). ( G ) Quantified PAS scores. ( H ) Representative Masson trichrome-stained lung tissues sections (scale bars: 200 μm and 50 μm, respectively). ( I ) Percentage of collagen fiber content in airway tissues. (n = 6). **P<0.01, ***P<0.001, ****P<0.0001.

Article Snippet: The concentration of total IgE in mouse serum was quantified using a commercial Mouse IgE ELISA Kit (Dakewe Biotech, China) according to the manufacturer’s instructions.

Techniques: Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Staining

Journal: JHEP Reports

Article Title: TAF15 in tumor-associated macrophages enhances protumorigenic polarization and promotes cholangiocarcinoma progression

doi: 10.1016/j.jhepr.2025.101545

Figure Lengend Snippet: TAF15 facilitates M2-like polarization of macrophages, enhancing TGFBI secretion and promoting the progression of ICC. (A) Reactome enrichment analysis of DEGs is depicted, with the top 10 enriched terms displayed. (B) Expression heatmaps of extracellular matrix organization. (C) Upper: a UMAP plot is colored by different clusters. Lower: a dot plot represents the mean expression of TGFBI across various clusters. (D) Western blot analysis examines the expression of TGFBI in different groups. (H) The colony formation ability of ICC cells treated with 0, 5, and 10 μg/ml recombinant TGFBI protein is assessed. (F–I) ELISA analysis measures the levels of TGFBI in the different CM of TAMs. Data are presented as mean ± SD, one-way ANOVA was performed for statistical analysis, n = 3. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. CM, conditional media; DEGs, differentially expressed genes; ICC, intrahepatic cholangiocarcinoma; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.

Article Snippet: The transforming growth factor-beta induced (TGFBI) ELISA test kit was purchased from CUSABIO (CSB-EL023450MO, CSB-E16665h, Wuhan, China).

Techniques: Expressing, Western Blot, Recombinant, Enzyme-linked Immunosorbent Assay, Binding Assay

Journal: JHEP Reports

Article Title: TAF15 in tumor-associated macrophages enhances protumorigenic polarization and promotes cholangiocarcinoma progression

doi: 10.1016/j.jhepr.2025.101545

Figure Lengend Snippet: M2pepLNP-siTaf15 shows promise in targeting macrophages for the treatment of ICC. (A,B) In vivo and in vitro small animal imaging was conducted using free IR780 and M2pepLNP/IR780 in ICC mice via the tail vein. (C) Self-luminescence (Dil) with F4/80 multicolor immunofluorescence. (D,E) Western blot and QPCR analysis of Taf15 in BMDM after transfection with small interference for 48 h. (F) A schematic diagram illustrates the encapsulation of siTaf15 by M2pep-liposomes. (G) A schematic diagram depicts the treatment regimen of ICC mice with liposome materials. (H) Differential morphology of the liver is observed after treatment with M2pepLNP coated with siNc and siTaf15. (I) Weight of the liver is measured following treatment with M2LNP coated with siNc and siTaf15. (J) H&E and IHC analyses are conducted for CK19 and TAF15 in liver tissues treated with M2pepLNP-siNc and M2pepLNP-siTaf15. (K) H&E analysis of heart, lung, spleen, kidney in mice treated with M2pepLNP-siNc and M2pepLNP-siTaf15. (L) Flow cytometry analysis the expression of CD206 and CD86 of macrophage in mice liver. (M) ELISA analysis measures the levels of TGFBI in eyeball blood and liver tissue from mice. Data are presented as mean ± SD, unpaired two-tailed Student’s t test. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. BMDM, bone marrow-derived macrophages; ICC, intrahepatic cholangiocarcinoma; IHC, immunohistochemistry; QPCR, quantitative PCR; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.

Article Snippet: The transforming growth factor-beta induced (TGFBI) ELISA test kit was purchased from CUSABIO (CSB-EL023450MO, CSB-E16665h, Wuhan, China).

Techniques: In Vivo, In Vitro, Imaging, Immunofluorescence, Western Blot, Transfection, Encapsulation, Liposomes, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Derivative Assay, Immunohistochemistry, Real-time Polymerase Chain Reaction, Binding Assay